traccuracy.loaders

Subpackage for loading tracking data into memory

This subpackage contains functions for loading ground truth or tracking method outputs into memory as TrackingGraph objects. Each loading function must return one TrackingGraph object which has a track graph and optionally contains a corresponding segmentation.

Package Contents

Functions

load_ctc_data(→ traccuracy._tracking_graph.TrackingGraph)

Read the CTC segmentations and track file and create a TrackingGraph.

load_tiffs(→ numpy.ndarray)

Load a directory of individual frames into a stack.

load_geff_data(→ traccuracy._tracking_graph.TrackingGraph)

Load a graph into memory from a geff file

load_point_data(, time_column, seg_id_column, name, sep)

Load point-based tracking data into a TrackingGraph from a csv-like file

traccuracy.loaders.load_ctc_data(data_dir: str, track_path: str | None = None, name: str | None = None, run_checks: bool = True) traccuracy._tracking_graph.TrackingGraph[source]

Read the CTC segmentations and track file and create a TrackingGraph.

Parameters:
  • data_dir (str) – Path to directory containing CTC tiffs.

  • track_path (optional, str) – Path to CTC track file. If not passed, finds *_track.txt in data_dir.

  • name (optional, str) – Name of data to store in TrackingGraph

  • run_checks (optional, bool) – If set to True (default), runs checks on the data to ensure valid CTC format.

Returns:

TrackingGraph object containing segmentations and graph.

Return type:

traccuracy.TrackingGraph

Raises:

ValueError – If the tracks file is not found. If run_checks is True, whenever any of the CTC format checks are violated. If run_checks is False, whenever any other Exception occurs while creating the graph.

traccuracy.loaders.load_tiffs(data_dir: str) numpy.ndarray[source]

Load a directory of individual frames into a stack.

Parameters:

data_dir (str) – Path to directory of tiff files

Raises:

FileNotFoundError – No tif files found in data_dir

Returns:

4D array with dims TYXC

Return type:

np.array

traccuracy.loaders.load_geff_data(geff_path: str, load_geff_seg: bool = False, seg_path: str | None = None, seg_property: str | None = None, name: str | None = None, load_all_props: bool = False) traccuracy._tracking_graph.TrackingGraph[source]

Load a graph into memory from a geff file

Segmentations can be optionally loaded either from a related object specified in the geff (load_geff_seg=True) or with a path to a zarr array seg_path and seg_property

Parameters:
  • geff_path (str) – Path to a geff group inside of a zarr,

  • load_geff_seg (bool, optional) – Load segmentation based on a geff metadata of related segmentation. Defaults to False.

  • seg_path (str | None, optional) – Path to a zarr array containing segmentation data. We assume that the axes order in your segmentation array matches the axes in your geff. If this is not true please load the segmentation yourself and add it to TrackingGraph.segmentation. Defaults to None.

  • seg_property (str | None, optional) – If seg_path provided, this is the corresponding property on the geff graph that contains the segmentation key. Defaults to None.

  • name (str | None, optional) – Optional name to store on TrackingGraph for identification. Defaults to None.

  • load_all_props (bool, optional) – If True, load all node and edge properties on the graph. Defaults to False and only spatiotemporal and segmentation node properties are loaded.

traccuracy.loaders.load_point_data(path: str | None = None, df: pandas.DataFrame | None = None, parent_column: str = 'parent', id_column: str = 'node_id', pos_columns: tuple[str, Ellipsis] = ('z', 'y', 'x'), time_column: str = 't', seg_id_column: str | None = None, name: str | None = None, sep: str | None = None) traccuracy._tracking_graph.TrackingGraph[source]

Load point-based tracking data into a TrackingGraph from a csv-like file

Assumes each row contains:

  • time

  • position, e.g. three columns ‘z’, ‘y’, ‘x’

  • parent, a reference to the node in the previous time frame. A node without a parent can be indicated by -1

Parameters:
  • path (str | None, optional) – Path to the csv-like file to load. Defaults to None.

  • df (pd.DataFrame | None, optional) – A dataframe that has already been loaded. Defaults to None.

  • parent_column (str | None, optional) – A reference to the parent node in the previous time frame. Defaults to “parent”.

  • id_column (str, optional) – Column used to specify node ids. Node IDs should be unique positive integers. Defaults to ‘node_id’

  • pos_columns (tuple[str], optional) – A tuple of columns to use for position. Defaults to (“z”, “y”, “x”).

  • time_column (str, optional) – The column to use for time. Defaults to “t”.

  • seg_id_column (str | None, optional) – Name of an optional column containing a segmentation label id. Defaults to None.

  • name (str | None, optional) – Optional string to name/describe the dataset. Defaults to None.

  • sep (str | None, optional) – Passed to pd.read_csv to set the sep kwarg. Defaults to None.

Raises:
  • ValueError – Must provide either a path or a dataframe

  • ValueError – parent_column not present in data

  • ValueError – id_column not present in data

  • ValueError – id_column does not contain positive integers

  • ValueError – id_column does not contain unique values

  • ValueError – pos_columns not present in data

  • ValueError – time_column not present in data

  • ValueError – seg_id_column not present in data

Returns:

TrackingGraph